A role for CTCF and cohesin in subtelomere chromatin organization, TERRA transcription, and telomere end protection
Identifieur interne : 002408 ( Main/Exploration ); précédent : 002407; suivant : 002409A role for CTCF and cohesin in subtelomere chromatin organization, TERRA transcription, and telomere end protection
Auteurs : Zhong Deng [États-Unis] ; Zhuo Wang [États-Unis] ; Nick Stong [États-Unis] ; Robert Plasschaert [États-Unis] ; Aliah Moczan [États-Unis] ; Horng-Shen Chen [États-Unis] ; Sufeng Hu [États-Unis] ; Priyankara Wikramasinghe [États-Unis] ; Ramana V. Davuluri [États-Unis] ; Marisa S. Bartolomei [États-Unis] ; Harold Riethman [États-Unis] ; Paul M. Lieberman [États-Unis]Source :
- The EMBO Journal [ 0261-4189 ] ; 2012-11-05.
English descriptors
- Teeft :
- Annu, Assay, Average value, Azzalin, Binding factors, Binding sites, Biol, Biology organization, Biology organization chromatin organization, Blasco, Canudas, Cell biol, Cell lines, Cell types, Centromeric, Chromatin, Chromatin organization, Chromatin structure, Chromosome, Cohesin, Cohesin subunit, Cohesin subunits, Ctcf, Ctcf binding, Ctcf depletion, Ctcf site, Ctcf sites, Ctcfbinding sites, Damage foci, Damage response, Data sets, Ddct methods, Deng, Depletion, Embo, Embo journal, Gene expression, Genet, Genome, Heterochromatin, Histone, Histone patterns, Human genome, Human subtelomeres, Human telomeres, Lentiviral, Lieberman, Luciferase, Luciferase activity, Mammalian telomeres, Mutation, Ndings, Nergadze, Nucleic acids, Plasmid, Point mutations, Polymerase, Primer, Primer sets, Promoter, Promoter activity, Promoter region, Relative luciferase activity, Reporter plasmid, Riethman, Rnapii, Rnapii binding, Rnapii recruitment, Room temperature, Shcontrol, Shctcf, Shelterin, Shrna, Sicontrol, Sirna, Smc1, Smc3, Subtelomere, Subtelomeres, Subtelomeric, Subunit, Supplementary figure, Supplementary figures, Supplementary table, Telomere, Telomeric, Transcription, Transcription regulation, Transcriptional, Transfected, Transfected cells, Trf1, Trf2, Trf2 binding, Ttaggg, U2os, U2os cells, U2os cells transduced, U2os cells transfected, Western blot.
Abstract
The contribution of human subtelomeric DNA and chromatin organization to telomere integrity and chromosome end protection is not yet understood in molecular detail. Here, we show by ChIP‐Seq that most human subtelomeres contain a CTCF‐ and cohesin‐binding site within ∼1–2 kb of the TTAGGG repeat tract and adjacent to a CpG‐islands implicated in TERRA transcription control. ChIP‐Seq also revealed that RNA polymerase II (RNAPII) was enriched at sites adjacent to the CTCF sites and extending towards the telomere repeat tracts. Mutation of CTCF‐binding sites in plasmid‐borne promoters reduced transcriptional activity in an orientation‐dependent manner. Depletion of CTCF by shRNA led to a decrease in TERRA transcription, and a loss of cohesin and RNAPII binding to the subtelomeres. Depletion of either CTCF or cohesin subunit Rad21 caused telomere‐induced DNA damage foci (TIF) formation, and destabilized TRF1 and TRF2 binding to the TTAGGG proximal subtelomere DNA. These findings indicate that CTCF and cohesin are integral components of most human subtelomeres, and important for the regulation of TERRA transcription and telomere end protection.
ChIP‐seq analyses reveal a common subtelomeric CTCF‐ and cohesion‐binding site important for RNA polymerase recruitment and TERRA transcription.
Url:
DOI: 10.1038/emboj.2012.266
Affiliations:
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Here, we show by ChIP‐Seq that most human subtelomeres contain a CTCF‐ and cohesin‐binding site within ∼1–2 kb of the TTAGGG repeat tract and adjacent to a CpG‐islands implicated in TERRA transcription control. ChIP‐Seq also revealed that RNA polymerase II (RNAPII) was enriched at sites adjacent to the CTCF sites and extending towards the telomere repeat tracts. Mutation of CTCF‐binding sites in plasmid‐borne promoters reduced transcriptional activity in an orientation‐dependent manner. Depletion of CTCF by shRNA led to a decrease in TERRA transcription, and a loss of cohesin and RNAPII binding to the subtelomeres. Depletion of either CTCF or cohesin subunit Rad21 caused telomere‐induced DNA damage foci (TIF) formation, and destabilized TRF1 and TRF2 binding to the TTAGGG proximal subtelomere DNA. These findings indicate that CTCF and cohesin are integral components of most human subtelomeres, and important for the regulation of TERRA transcription and telomere end protection.</div><div type="abstract">ChIP‐seq analyses reveal a common subtelomeric CTCF‐ and cohesion‐binding site important for RNA polymerase recruitment and TERRA transcription.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Pennsylvanie</li></region><settlement><li>Philadelphie</li></settlement></list><tree><country name="États-Unis"><region name="Pennsylvanie"><name sortKey="Deng, Zhong" sort="Deng, Zhong" uniqKey="Deng Z" first="Zhong" last="Deng">Zhong Deng</name></region><name sortKey="Bartolomei, Marisa S" sort="Bartolomei, Marisa S" uniqKey="Bartolomei M" first="Marisa S" last="Bartolomei">Marisa S. Bartolomei</name><name sortKey="Chen, Horng Hen" sort="Chen, Horng Hen" uniqKey="Chen H" first="Horng-Shen" last="Chen">Horng-Shen Chen</name><name sortKey="Davuluri, Ramana V" sort="Davuluri, Ramana V" uniqKey="Davuluri R" first="Ramana V" last="Davuluri">Ramana V. Davuluri</name><name sortKey="Hu, Sufeng" sort="Hu, Sufeng" uniqKey="Hu S" first="Sufeng" last="Hu">Sufeng Hu</name><name sortKey="Lieberman, Paul M" sort="Lieberman, Paul M" uniqKey="Lieberman P" first="Paul M" last="Lieberman">Paul M. Lieberman</name><name sortKey="Moczan, Aliah" sort="Moczan, Aliah" uniqKey="Moczan A" first="Aliah" last="Moczan">Aliah Moczan</name><name sortKey="Plasschaert, Robert" sort="Plasschaert, Robert" uniqKey="Plasschaert R" first="Robert" last="Plasschaert">Robert Plasschaert</name><name sortKey="Riethman, Harold" sort="Riethman, Harold" uniqKey="Riethman H" first="Harold" last="Riethman">Harold Riethman</name><name sortKey="Stong, Nick" sort="Stong, Nick" uniqKey="Stong N" first="Nick" last="Stong">Nick Stong</name><name sortKey="Wang, Zhuo" sort="Wang, Zhuo" uniqKey="Wang Z" first="Zhuo" last="Wang">Zhuo Wang</name><name sortKey="Wikramasinghe, Priyankara" sort="Wikramasinghe, Priyankara" uniqKey="Wikramasinghe P" first="Priyankara" last="Wikramasinghe">Priyankara Wikramasinghe</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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